Long-term Steady State Perfusion Culture of Mammalian Cells Using a Robust Microfluidic Cell Retention Device
نویسندگان
چکیده
Cell retention devices are used to retain cells in containers during perfusion culture. However, the conventional membrane-based filtration devices have challenges such as membrane fouling/clogging and increased contamination risk due to frequent filter replacements. To solve these challenges, we introduce a new microfluidic cell retention device based on inertial cell focusing. We demonstrated a long-term steady state perfusion culture of suspended CHO cells, where high density of cells (> 3×10 6 cells/mL) and viability (> 90%) were maintained for more than a week. Our membrane-less and clog-free cell retention device has unique advantage over the conventional filtration devices for perfusion culture.
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